Maleficient, Cersei Lannister, Hela, Bellatrix Lestrange, Cruella, Ursula, Queen of Heart, Poison Ivy, Wicked Witch, Mom from Futurama, not sure of the xenomorph queen from Aliens counts?, GLaDOS, Number Six, Stargate had a few over the years, Starcraft’s Kerrigan/Queen of blades, Resident Evil’s Lady Dimetrescu, Carmen Sandiego, Doom’s Olivia Pierce, Airbender’s Azula, Carrie, Annie Wilkes from Misery, Claire from House of Cards, the Aunt in Handmaiden, Harley Quinn, Lady Macbeth…